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The ioLight Compact Cell Imager lineup consists of inverted micro-imaging devices engineered for incubator, safety cabinet, and benchtop cell culture monitoring. Featuring a 1.0 µm optical resolution (NA 0.25, equivalent to a 10x objective) and an approximate 1.2 x 0.9 mm field of view, each unit captures 5 MP color still images (2592 x 1944 pixels) and MPEG-4 video. They support multiple transmitted illumination modes, including Brightfield, Darkfield, and Digital Phase Contrast. Designed to fit inside standard CO2 incubators (210 x 140 x 132 mm footprint, 1.8 kg), they feature sealed power cables and operate reliably up to 90% non-condensing relative humidity. All models accommodate standard Petri dishes, well plates, and flasks up to 48 mm in height, connecting wirelessly via integrated Wi-Fi to mobile or desktop browsers for remote image acquisition, Z-stacking, and time-lapse recordings. The iolight Compact Cell Imager with Fluorescence is an engineered epifluorescence module underneath the sample stage (featuring a 465 nm excitation source and a 500–700 nm emission detection window). It retains all standard trans-illumination capabilities while adding targeted fluorophore tracking. This allows researchers to identify common markers such as GFP, YFP, Propidium Iodide (PI), SYTO9, and DsRed to quantify transfection efficiencies or monitor cell viability and structural differentiation in real time.
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